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Structured Review

Merck KGaA dna fragment pet3a
Dna Fragment Pet3a, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+fragment+pet3a/pet3a+vector/pmc10245121-314-7-12
Average 90 stars, based on 1 article reviews
dna fragment pet3a - by Bioz Stars, 2026-11
90/100 stars

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Amplification:

Article Title: GH20 and GH84 β- N -acetylglucosaminidases with different linkage specificities underpin mucin O -glycan breakdown capability of Bifidobacterium bifidum
Article Snippet: A DNA fragment encoding 29 to 1934 amino acid residues of BbhIV was PCR-amplified using KOD-One (Toyobo) with the genomic DNA of B. bifidum JCM 1254 as a template. .. The amplified fragment was ligated with a DNA fragment amplified from pET3a (Merck-Millipore), which generated an expression plasmid pET3a-BbhIV-His 6 . ..

Generated:

Article Title: GH20 and GH84 β- N -acetylglucosaminidases with different linkage specificities underpin mucin O -glycan breakdown capability of Bifidobacterium bifidum
Article Snippet: A DNA fragment encoding 29 to 1934 amino acid residues of BbhIV was PCR-amplified using KOD-One (Toyobo) with the genomic DNA of B. bifidum JCM 1254 as a template. .. The amplified fragment was ligated with a DNA fragment amplified from pET3a (Merck-Millipore), which generated an expression plasmid pET3a-BbhIV-His 6 . ..

Expressing:

Article Title: GH20 and GH84 β- N -acetylglucosaminidases with different linkage specificities underpin mucin O -glycan breakdown capability of Bifidobacterium bifidum
Article Snippet: A DNA fragment encoding 29 to 1934 amino acid residues of BbhIV was PCR-amplified using KOD-One (Toyobo) with the genomic DNA of B. bifidum JCM 1254 as a template. .. The amplified fragment was ligated with a DNA fragment amplified from pET3a (Merck-Millipore), which generated an expression plasmid pET3a-BbhIV-His 6 . ..

Plasmid Preparation:

Article Title: GH20 and GH84 β- N -acetylglucosaminidases with different linkage specificities underpin mucin O -glycan breakdown capability of Bifidobacterium bifidum
Article Snippet: A DNA fragment encoding 29 to 1934 amino acid residues of BbhIV was PCR-amplified using KOD-One (Toyobo) with the genomic DNA of B. bifidum JCM 1254 as a template. .. The amplified fragment was ligated with a DNA fragment amplified from pET3a (Merck-Millipore), which generated an expression plasmid pET3a-BbhIV-His 6 . ..



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90
Merck KGaA dna fragment pet3a
Dna Fragment Pet3a, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+fragment+pet3a/pet3a+vector/pmc10245121-314-7-12
Average 90 stars, based on 1 article reviews
dna fragment pet3a - by Bioz Stars, 2026-11
90/100 stars
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